Email this article 
Hide
Show all
The Role of Tannins as Plant Based Nematicide for Management of Root-Gall Nematode in Okra (Abelmoschus esculentus)
Abstract
In vitro and screen house studies were conducted to investigate the effectiveness of physicnut tannins and plant parts against root-gall nematode infected okra. The effectiveness of tannins against nematode juveniles (J2s) mortality was evaluated in the laboratory. Data was collected on percentage mortality at 24, 48 and 72 h incubation period. Root extracts of tannins caused 94% mortality after 72 hours at a 10 ml application rate. Results obtained under screen house condition showed that leaf and seed extracts of tannins applied at 5 ml/pot and inoculated with 1,200 infective nematode larvae (J2s) significantly (P=0.05) decreased root-gall index from 4.00 (severely galled) to 1.00 (rarely galled) while enhancing growth and yield attributes of okra than the untreated control. Maximum effect of treatment rates on root gall index and yield was most impactful at the highest dose of 5ml/pot. Correlation of root-gall index and yield attributes substantiated the effectiveness of tannins in improving the growth and yield of Okra at reduced galling responses. While leaf and seed tannins application were most effective in increasing plant heights, leaf areas and okra pod weights by 67% (4.88 g) over the control, highest 100 dry seed weight and improved mucilaginous property were achieved with root tannins application at 5ml. Utilizing crude tannins extracts with well-established bioactive principles can therefore serve as eco-friendly alternative nematicide and help mitigate the harmful effects that synthetic nematicides have on flora and fauna.
Full Text:
PDFReferences
Adegbite, A. A. and Adesiyan, S.O. 2005. Root extracts of plants to control Root-knot Nematodeon edible Soybean. World Journal of Agricultural Sciences, 1(1): 18-21.
Agu, C.M and R.O. Ogbuji. 1996. Soybean resistance to the root-gall nematode, as influenced by Potassium nutrition. East African Agricultural Journal 66(3): 273-276. https://www.africabib.org/rec.php?RID=Q00049962
Agu, C.M., A.C. Nwagbaga, C.G. Uwandu, P. Onoh, C.A. Mbuka, F.O. Ojiako, N.K. Ndulue and V.I Ogwudire. 2013. Effect of Jatropha Curcas on Root-gall Nematode Disease and the Consequent Proximate Composition of Okra. Bionature Journal of India, 33 (2): 59-62. https://globalpresshub.com/index.php/BN/article/view/562
Agu, C.M., C.O. Mbuka and V.E. Ogwudire. 2009. Influence of root-gall nematode on mucilaginous property and nutrient composition of Okra (Abelmoschus esculentus L. Moench). Bionature, 29(2): 73-77. https://globalpresshub.com/index.php/BN/article/view/447
Akinmoladun, A.C., E.O. Ibukun, E. Afor, E.M. Obuotor and E.O Farombi. 2007. Phytochemical Constituents and Antioxidant Activity of Extract from the Leaves of the Ocimum graticimum. Scientific Research Essay, 2: 163-166. http://www.academicjournals.org/SRE.
Alam, E.A. and A.S.E. Nuby. 2022. Phytochemical and nematicidal screening on some extracts of different plant parts of Egyptian Moringa oleifera L. Pakistan Journal of Phytopathology, 34(2): 293-306.
Anwar, S.A., M.V and McKenry. 2010. Incidence and reproduction of Meloidogyne incognita on vegetable crop genotypes. Pakistan Journal of zoology, 42(2):135-141.
Atiq, M., L. Malik, N.A. Rajput, M. Shabbir, M. Ashiq, M.J. Matloob, A. Waseem, A. Jabbar, A. Ullah, R. Zawar and A. Majeed. 2024. Evaluating the efficacy of plant-mediated copper-silver nanoparticles for controlling Cercospora leaf spot in mung beans. Plant protection, 08(04).
Azhagumurugan., C and M.K. Rajan. 2014. Extract of Nilakumil, (gmelina asiatica) against the Root Knot Nematode, (Meloidogyne incognita). Research Journal of Recent Sciences. Vol. 3(ISC-2013), 264-266 ISSN 2277-2502. www.isca.in.
Castillo, P.D.I., M. Vito, N. Vovlas and R.M. Jiménez-Díaz. 2001. Host-parasite relationships in root-knot disease of white mulberry. Plant Disease, 85: 277-281.
Chitwood, D.J. 2002. Phytochemical Based Strategies for Nematode Control. Annuual Review of Phytopathology, 40:221–49.doi: 10.1146/annurev.phyto.40.032602.130045
Devappa, R.K., H.P.S. Makkar and K. Becker. 2010. Jatropha toxicity- a review Journal of Toxicology and Environmental Health, Part B, 13: 476-507. doi: 10.1080/10937404.2010.499736.
Eisenback, J.D., H. Hirschmann, J.N. Sasser and A.C. Triantaphyllou. 1981. A guide to the four most common species of root-knot nematodes (Meloidogyne spp) with a pictorial key. A cooperative publication of North Carolina State University and USAID. pp: 48. https://pdf.usaid.gov/pdf_docs/pnaaq221.pdf
Hong, L., G. Li, W. Zhou, X. Wang and K. Zhang. 2007. Screening and isolation of a nematicidal sesquiterpene from Magnolia grandiflora. Pest Management Science, 63: 301-305. doi: 10.1002/ps.1337.
Humaira – Shad, A.A., D. Muhammad and H.U. Shah. 2020. Evaluation of Some Medicinal Plant Extracts for Their Nematicidal Properties Against Root-Knot Nematode, Meloidogyne incognita. Applied Ecology and Environmental Research 18(2):2475-2482. http://www.aloki.hu. ISSN 1589 1623 (Print). ISSN1785 0037 (Online) DOI: http://dx.doi.org/10.15666/aeer/1802_24752482
Igbinosa, O.O., E.O. Igbinosa and O.A. Aiyegoro. 2009. Antimicrobial Activity and Phytochemical Screening of Stem Bark Extracts from Jatropha curcas (Linn). African Journal of Pharmacy and Pharmacology, 3 (9): 58-62. www.internationalscholarsjournals.org
Janathan, E.I and G. Rajendran. 2002. Assessment of avoidable yield loss in banana due to root-knot nematode Meloidogyne incognita. Indian Journal Nematology, 30: 162-164. https://www.cabdirect.org/cabdirect/abstract/20023059279
Khan, A., M. Asif, M. Tariq, B. Rehman, K. Parihar and M.A. Siddiqui. 2017. Phytochemical investigation, nematostatic and nematicidal potential of weeds extract against the root-knot nematode, Meloidogyne incognita in vitro. Asian Journal of Biological Science, 10: 38-46.
Maher, S., N. Naheed, N. Khan, H. Ishtiaq, G. S. Solangi, A. karim, M. Imran, E. Iqbal, F. Mukhtar, M. Bakhshi, G. Mina and S. S. Alarfaji. 2024. Phytochemical Analysis and Nematicidal Activity of Medicinal Plants Against Meloidogyne javanica. Bio Rxiv, doi:https://doi.org/10.1101/2024.09.29.615697.
Maistrello, L., Vaccari, G and N. Sasanelli. 2010. Effect of chestnut tannins on the root-knot nematode Meloidogyne javanica Parasitological Institute of SAS, Helminthologia, 47(1): 48 – 57. DOI 10.2478/s11687-010-0008-9
Mba, O.I., N.C. Ohazuruike and E.A. Okorocha. 2005. An assessment of the nematicidal potential of some plant products in the control of root-knot nematode (M. incognita, Kofoid and White) on tomato plant. Procceedings of the 32nd Annual Conference of Nigeria Society for Plant Protection. 15th – 20th May 2005. University of Port Harcourt, Nigeria, 37-41.
Molan, A.L. 2014. Effect of purified condensed tannins from pine bark on larval motility, egg hatching and larval development of Teladorsagia circumcincta and Trichostrongylus colubriformis (Nematoda: Trichostrongylidae). Folia Parasitol (Praha). 61: 371–6. doi: 10.14411/fp.2014.036.
Mukhtar, T., A. Israr, M. Zameer, H.M. Arshad, S.B. Kayani, R.A. Murad and A. Muhammad. 2013. Estimation of Damage to Okra by Root-Knot Disease Mated by Meloidogyne incognita. Pakistan Journal of Botany, 45 (3): 1023 – 1027. https://www.researchgate.net/publication/236975524
Naz, I., Saifullah, M.R and Khan. 2013. Nematicidal activity of nonacosane-10-ol and 23a-Homostigmast-5-en-3β-ol isolated from the roots of Fumaria parviflora (Fumariaceae). Journal of Agricultural and Food Chemistry, vol. 61, no. 24, pp. 5689-5695. http://dx.doi.org/10.1021/jf401309r. PMid:23713689.
Ojiako, F.O., G.O. Ihejirika and U.O. Aguwa. 2015. Comparative bioactivity of different solvent extracts of the root and seeds of Jatropha curcas L. and Chlorpyrifos, against tailor ants (Oecophylla longinoda Latreille) (Hymenoptera: Fo rmicidae). FUTO Journal Series (FUTOJNLS).1 (2); p-ISSN: 2467-8325; e-ISSN: 2476-8456. https://www.researchgate.net/publication/298972376_
Onkendi, E.M., G.M. Kariuki, M. Mara and L.N. Moleleki. 2014. The threat of root-knot nematodes (Meloidogyne spp.) in Africa: a review. Forestry Agriculture and Biotechnology Institute (FABI). Department of Microbiology and Plant Pathology. University of Pretoria. South Africa.doi:10.1111/ppa.12202
Parveen, K., A. Haseeb and P.K.I. Shukla. 2006. Pathogenic potential of Meloidogyne incognita on Mentha arvensis cv. Gomti. Indian Journal of Nematology, 36: 177-180. https://www.indianjournals.com/ijor.aspx?target=ijor:ijn&volume=36&issue=2&article=001
Siji, J.V., C.A. Jayaprakas, M.S. Sheela and C. Mohandas. 2010. Efficacy of Cleome viscosa L. against Meloidogyne incognita infestation in okra (Abelmoschus esculentus L.). Thai Journal of Agricultural Science, 43 (3): 151-156. https://www.thaiscience.info/Journals/Article/TJAS/10656559.pdf
Sopade, P., Ajisegiri, E and Badau. 1992. The Use of Peleg’s Equation to Model Water Absorption in some Cereal Grains during Soaking. Journal of Food Engineering, 15:269-283.
SP-IPM, 2000. Biological alternatives to harmful chemical pesticide. IPM Research brief no. 4. The Systemwide Program on Integrated Pest Management for improved livelihood. www.spipm.cgiar.org.
Strumeyer, D.H and M.J. Malin.1975. Condensed Tannins in grain sorghum Isolation, Fraction, and Characterization. Journal of Agricultural and Food Chemistry, 23 (5): 909-914. doi:10.1021/jf60201a019
Tariq-Khan, M. S.Z.A. Gardazi, A.D.A. Khan, M. Iyas and M. Ahmad. 2020. Virulence and distribution trends of root-knot nematode (RKN) fauna on summer vegetables in District Bagh, Azad Jammu and Kashmir (Pakistan). Pakistan Journal of Nematology, 38(2): 139-148. doi http://dx.doi.org/10.17582/journal.pjn/2020.38.2.139.148
Tong Z, W He, X Fan, A Guo, 2022 Biological Function of Plant Tannins and Its Application in Animal Health. Front. Vet. Sci. 8:803657. doi: 10.3389/fvets.2021.803657.
Ugwoke, K.I., B.O. Ukwueze and S.I. Ogwulumba. 2011. Powdery leaf extracts for control of root-knot nematode in African Yam Bean. African Crop Science journal. Vol. 19, No. 2, pp. 13
Ukoha P.O., A.C. Egbuonu, Cemaluk, L. Obasi, Nnamdi, P.M Ejikeme. 2011. Tannins and other phytochemical of the Samanaea saman pods and their antimicrobial activities. African Journal of Pure and Applied Chemistry, 5(8): 237-244, http://www.academicjournals.org/AJPAC ISSN 1996 - 0840.
Usman, M., M. Atiq, N.A. Rajput, S.T. Sahi, M. Shad, N. Lili, S. Iqbal, A.M. Arif, U. Ahmad, K.S. Khan, M. Asif, F.U. Haider. 2024. Efficacy of Green Synthesized Silver Based Nanomaterials against Early Blight of Tomato Caused by Alternaria solani. Gesunde Pflanzen. 1-11.
DOI: https://doi.org/10.33866/phytopathol.036.02.1154
Refbacks
- There are currently no refbacks.
Copyright (c) 2024 VINCENT EZECHUKWU OGWUDIRE
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
 |  |  | ||
| Pakistan Journal of Phytopathology ISSN: 1019-763X (Print), 2305-0284 (Online). © 2013 Pak. J. Phytopathol. All rights reserved. | ||||