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First Detection of Candidatus phytoplasma asteris in Papaya Orchards in Ivory Coast
Abstract
Papaya is an important food and economic crop in Ivory Coast. However, its cultivation is facing persistent virus problems. Recent studies carried out around the world have shown that phytoplasmas cause symptoms similar to viruses and are increasingly affecting various crops. Thisstudywascarriedoutwiththeaimofidentifyingthephytoplasma(s)likelytobeassociated with leaf deformation symptoms in papaya trees in Ivory Coast. Leaf deformation symptoms wereobservedonplantsofthesolo.Phytoplasmasweredetectedinleafsamplescollectedfrom December 2022 to February 2023 by nested polymerase chain reaction with primer pairs amplifying the 16Sr phytoplasma genes. The results showed a wide range of leaf symptoms. The prevalence of foliar symptoms varied from 1.85% to 60%. Sequencing of the amplicons and phylogenetic analysis revealed that Candidatus phytoplasma asteris of the 16SrI-B subgroup was the phytoplasma detected in 11 leaf samples out of 38 (28.94%) using NestedPCRcarriedoutwiththeR16F2n/R16R2primer.However,notracesofthephytoplasma responsibleforthefatalcoconutyellowingdisease,whichaffectscoconutandmaniocinIvory Coast,werefoundinthesesamples.Theresultsobtainedhaveimprovedthediagnosisofpapaya diseases in Ivory Coast, as they have made it possible to associate an agent other than viruses and fungi, which have always been associated with papaya infections in all the studies carried out in Ivory Coast.
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DOI: https://doi.org/10.33866/phytopathol.036.02.1207
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