DETECTION OF SEED TO SEED TRANSMISSION OF BOTRYTIS CINEREA IN LETTUCE

Elias N.K.Sowley

Abstract


Botrytis cinerea is pathogenic to many crops including lettuce. The aim of this study was to test whether B. cinerea contaminated seed could give rise to infected plants and whether infected plants could produce infected seed as a means of confirming the endophytic life cycle. B. cinerea was detected in seed and within plants both by isolation on Botrytis selective medium (BSM) and by qPCR, with and without prior surface sterilisation. B. cinerea was detected more frequently by qPCR (P<0.001) and isolation on BSM in cv. Diana and cv. Tom Thumb respectively. Within growing plant stems qPCR detected more B. cinerea while in roots and leaves more of the pathogen was recovered on BSM. The detection of B. cinerea in lettuce seedlings grown from infected seed in isolation is evidence of seed to seedling transmission while detection of infection in seeds harvested from plants which were grown in the field and flower/bud inoculated proves that there was seedling to seed transmission.


Keywords


qPCR; seedborne; systemic; Taqman assay; transmission

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Pakistan Journal of Phytopathology
ISSN: 1019-763X (Print), 2305-0284 (Online).
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