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ANTIMICROBIAL POTENTIAL OF FEMALE CONES AND NEEDLES OF CHIR PINE (PINUS ROXBURGHII SARGENT)
Abstract
The present study was undertaken to evaluate the antimicrobial potential of the plant Pinus roxburghii Sarg. The aerial parts of Pinus (needles and female cones) were extracted using various solvents viz. petroleum ether, chloroform, methanol and distilled water. These extracts were used for preliminary phyto chemical screening by using disc diffusion method and were tested against two fungi viz. Alternaria alternata & Fusarium solani and two bacteria viz. Xanthomonas oryzae & Pseudomonas alcaligenes. Results revealed that female cones of P. Roxburghii have more antimicrobial activity as compared to needles therefore, female cones extract in chloroform showed maximum inhibitory zones against fungus A. Alternate (22±2a) and methanolic extract against F. solani (20±2a). When comparing with P. alcaligenes, X.oryzae growth was more inhibited by cones and needle extracts. From results it may concluded that methanolic extract of cones and needles created maximum zones of inhibition against X.oryzaei.e.,23.3±2a and16±4a respectively and 22±2a. In case of P. alcaligenes, female cones methanolic extract showed maximum value of zone of inhibition i.e., 22±2a while needles showed maximum value in chloroform extract i.e., 15±0.35b. Therefore it can be declared that P. roxburghii is promising phyto medicine having strong antifungal and antibacterial activities.
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