MORPHOLOGICAL AND CULTURAL CHARACTERIZATION OF BOTRYTUS CINEREA CAUSING GRAY MOLD DISEASE OF LENTIL CROP FROM PAKISTAN

Nawab Zada, Gulshan Irshad, Farah Naz, Asim Gulzar, Muhammad Shahid, Muhammad F. Aslam, Salman Ghuffar

Abstract


The lentil (Lens culinaris) is an edible pulse crop and belongs to family Leguminosae. Fungal diseases of lentil are the most important biological constraint to productivity. Out of which Botrytis Gray mold causes significant losses. The present investigation is carried out to characterize Botrytis gray mold on lentil crop. During the survey 11 isolates of Botrytis Cinerea causing grey mold disease was collected different lentil growing areas of Pothwar regions. The isolates were identified on the basis of cultural and microscopic features. After 6 days during cultural studies maximum growth was observed on Czepk and Malt extract medium (85 mm) and minimum growth was on PDA (74.1 mm). The colony color was varies from whitish to black grayish with fluffy texture. Microscopically B. cinerea produces gray mycelium with branched conidiophores that that have rounded apical cells bearing cluster of colorless or gray one celled, ovoid conidia. The conidiophores and cluster of conidia resembles as a grape-like cluster. The pathogenecity test confirmed all the isolates of B. Cinerea were able to cause the gray mold disease. The overall objective of this study is to morphologically characterize the Botrytis cinera that provides a due step for management of Botrytis grey mould disease of lentil crops.


Keywords


Lentil; Botrytus cinerea; Isolation; Morphological and cultural characterization

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Copyright (c) 2016 Nawab Zada, Gulshan Irshad, Farah Naz, Asim Gulzar, Muhammad Shahid, Muhammad F. Aslam, Salman Ghuffar

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Pakistan Journal of Phytopathology
ISSN: 1019-763X (Print), 2305-0284 (Online).
© 2013 Pak. J. Phytopathol. All rights reserved.