COMPARATIVE EFFICACY OF FUNGICIDES AND SALICYLIC ACID AGAINST MACROPHOMINA PHASEOLINA CAUSING CHARCOAL ROT UNDER LABORATORY CONDITIONS

Habiba U. Rehman, Muhammad U. Ghazanfar, Muhammad S. Goraya

Abstract


To date, in Pakistan, no fungicide has been registered against Macrophomina phaseolina. This study was designed to check the efficacy of two fungicides and salicylic acid against M. phaseolina under laboratory conditions. Both fungicides and salicylic acid had given significant results inhibiting the mycelial growth compared to control. The application of Nativo @ 6 mM resulted maximum inhibition in mycelial growth at 3rd day (69.32%), at 5th day (70.84) and at 7th day (73.82%) than control. Fungal growth reduced with the increase in the concentration of fungicides and salicylic acid and decreased with decrease in concentration. The application of Success @ 6mM gave a good result but lower than the Nativo. Both the fungicides and Salicylic acid gave maximum inhibition of fungal growth at the concentration of 6mM. Mycelial growth recorded at 7th day with the treatment of salicylic acid @ 6mM was 55.04% as compared to the control followed by Success 70.23%. The results showed Nativo @ 6 mM may be the best for the control of charcoal rot under laboratory conditions.


Keywords


Sunflower, Charcoal rot, Macrophomina phaseolina

Full Text:

PDF

References


Aboshosha, S., S. Attaalla, A. El-Korany and E. El-Argawy. 2007. Characterization of Macrophomina phaseolina isolates affecting sunflower growth in El-Behera governorate, Egypt. International Journal of Agriculture and Biology, 9: 807-815.

Ataga, A. and C. Akueshi. 1986. Changes in oil and free fatty acid contents of Sunflower seeds inoculated with Alternaria tenuis Auct., Curvularia lunata (Wakker) Boedijn, Fusarium moniliforme Sheld. and Macrophomina phaseolina (Tassi) Goid. Phytopathologia Mediterranea, 25: 44-46.

Bashir, M. R., A. Mehmood, M. Sajid, M. A. Zeshan, M. Mohsin, Q. A. T. Khan and F. A. Tahir. 2017. Exploitation of new chemistry fungicides against charcoal rot of sesame caused by macrophomina phasiolina in Pakistan. Pakistan Journal of Phytopathology, 29: 257-263.

Chang, K. F., S. F. Hwang, H. Wang, G. Turnbull and R. Howard. 2006. Etiology and Biological Control of Sclerotinia Blight of Coneflower Using Trichoderma Species. Plant Pathology Journal, 5: 15-19.

Chennakesavulu, M., M. Reddikumar and N. E. Reddy. 2013. Evaluation of different fungicides and their compatibility with Pseudomonas fluorescens in the control of redgram wilt incited by Fusarium udum. Journal of Biological Control, 27: 354-361.

Crous, P. W., B. Slippers, M. J. Wingfield, J. Rheeder, W. F. O. Marasas, A. J. L. Philips, A. Alves, T. Burgess, P. Barber and J. Z. Groenewald. 2006. Phylogenetic lineages in the Botryosphaeriaceae. Study Mycology, 55: 235-253.

Dhingra, O. D. and J. B. Sinclair. 1977. An annotated bibliography of Macrophomina phaseolina 1905-1975.

Diourte, M., J. L. Starr, M. J. Jeger, J. P. Stack and D. T. Rosenow. 1995. Charcoal rot (Macrophomina phaseolina) resistance and the effects of water stress on disease development in sorghum. Plant Pathology, 44: 196-202.

FAO. 2017. Sunflower production in Pakistan, Food and Agricultural Organizarion. http://www.factfish.com/statistic-country/pakistan/sunflower+seed,+production+quantity. Assessed on 20.12.2019.

Ghaffar, A. 1988. Soil borne disease research center. Final research report. Department of Botany, University of Karachi 75270, Pakistan.: 111.

Gupta, G. K., S. K. Sharma and R. Ramteke. 2012. Biology, Epidemiology and Management of the Pathogenic Fungus Macrophomina phaseolina (Tassi) Goid with Special Reference to Charcoal Rot of Soybean (Glycine max (L.) Merrill). Journal of Phytopathology, 160: 167-180.

Iqbal, U. and T. Mukhtar. 2020. Evaluation of Biocontrol Potential of Seven Indigenous Trichoderma Species against Charcoal Rot Causing Fungus, Macrophomina phaseolina. Gesunde Pflanzen, 72: 195-202.

Islam, M. S., M. S. Haque, M. M. Islam, E. M. Emdad, A. Halim, Q. M. M. Hossen, M. Z. Hossain, B. Ahmed, S. Rahim, M. S. Rahman, M. M. Alam, S. Hou, X. Wan, J. A. Saito and M. Alam. 2012. Tools to kill: genome of one of the most destructive plant pathogenic fungi Macrophomina phaseolina. BMC Genomics, 13: 493-493.

Javaid, A. and M. Amin. 2009. Antifungal activity of methanol andn-hexane extracts of three Chenopodium species against Macrophomina phaseolina. Natural Product Research, 23: 1120-1127.

Kata, J. 2000. Physical and cultural methods for the management of soil-borne pathogens. Crop Protection, 19: 725-731.

Kataria, H. R. and R. K. Grover. 1976. Some factors affecting the control of Rhizoctonia solani by systemic and non-systemic fungicides. Annals of Applied Biology, 82: 267-278.

Khalili, E., M. A. Javed, F. Huyop, S. Rayatpanah, S. Jamshidi and R. A. Wahab. 2016. Evaluation of Trichoderma isolates as potential biological control agent against soybean charcoal rot disease caused by Macrophomina phaseolina. Biotechnology & Biotechnological Equipment, 30: 479-488.

Kumar, S., D. Rc and M. Dk. 2016. Biosurfactant-mediated Biocontrol of Macrophomina phaseolina Causing Charcoal Rot in Vigna mungo by a Plant Growth Promoting Enterococcus sp. BS13. Journal of Plant Pathology & Microbiology, 7: 2-8.

Markell, S. G., R. M. Harveson, C. C. Block and T. J. Gulya. 2015. Sunflower Diseases. Sunflower. Elsevier, 93-128.

Mirza, M. 1984. Occurrence of sunflower diseases in Pakistan in 1980-83. Proceedings of the National Sunflower Workshop, PARC. 31-32.

Mirza, M. and A. Beg. 1983. Diseases of sunflower in Pakistan-1982. Information bulletin of the FAO research network on sunflower Helia. 6: 55-56.

Parmar, H., H. Kapadiya and C. Bhaliya. 2017. Efficacy of different fungicides against Macrophomina phaseolina (Tassi) Goid causing castor root rot. International Journal of Communication Systems, 5: 1807-1809.

Sağir, P., A. Sağir and T. Söğüt. 2009. The Effect of Charcoal Rot Disease (Macrophomina phaseolina), Irrigation and Sowing Date on Oil and Protein Content of Some Sesame Lines. The Journal of Turkish Phytopathology, 38: 33-42.

Satish, L., G. Gupta and S. Saroj. 1986. Crop disease situation and some new records in Indian arid zone. Annals of Arid Zone, 25: 311-320.

Sireesha, O. and N. Venkateswarlu. 2013. In vitro evaluation of botanicals and panchagavya against leaf blast fungus Pyricularia grisea. Asian Journal of Pharmacy Clinic and Research, 6: 84-86.

Steel, R. and J. Torrie. 1997. Principle and Procedure of Statistics: A Biometrical Approach, p: 633. McGraw Hill Publication Co. New York.

Swamy, C., M. K. Naik, Y. S. Amaresh and S. K. Jayalakshmi. 2018. Evaluation of Fungicides and Bio-Agents under in vitro Condition against Macrophomina phaseolina Causing Stem Canker of Pigeonpea. International Journal of Current Microbiology and Applied Sciences, 7: 811-819.

Ullah, M. H., M. A. Khan, S. Sahi and A. Habib. 2011. Evaluation of antagonistic fungi against charcoal rot of sunflower caused by Macrophomina phaseolina (Tassi) Goid. African Journal of Environmental Science and Technology, 5: 616-621.




DOI: https://doi.org/10.33866/phytopathol.033.02.0609

Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Habiba Ur Rehman

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

      
   
Pakistan Journal of Phytopathology
ISSN: 1019-763X (Print), 2305-0284 (Online).
© 2013 Pak. J. Phytopathol. All rights reserved.