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SYNERGISM BETWEEN TRICHODERMA HARZIANUM RIFAI AND CHEMICAL FUNGICIDE FOR THE CONTROL OF FUSARIUM OXYSPORUM SCHLTDL. ON MUNG BEAN
Abstract
The Present study was carried out to find out the compatible dose of the fungicide with Trichoderma harzianum (TH) and the study also found way to utilize the synergy of the fungicide (Copper Hydroxide) and the fungal biocontrol agent against soil borne plant pathogen causing Fusarium wilt in Mung bean (Vigna Radiata L.). TH is a well known fungicidal biocontrol agent. The efficiency TH strain was studied against a soil borne plant pathogen Fusarium oxysporum (FO). The biocontrol strain TH was mainly observed under in-vitro assays was found to be compatible with the Copper Hydroxide, an agro-fungicide formulation sold with a trade name Kocide (KD); TH tolerated to the fungicide KD as the mycelia reduced 50% growth (EC50) at 176.1 ppm. KD increased the mycocidal potential of the TH at the highest tolerable dose (20 ppm < EC10 =58.2 ppm), from 24 to 26% against FO on food poisoned media, which was the evidence to the fungicide synergy (02%) with the biocontrol effects of the Under in vivo conditions, the synergy between KD and TH was found to be higher (26.1 %) in combating FO infection in mungbean plants in comparison to in vitro assay. Under in vivo conditions, the synergy between KD and TH was found to be higher (26.1 %) in combating FO infection in mungbean plants in comparison to in vitro assay. The combine use of KD and TH also positively impacted the plant growth where plants treated with both KD and TH had XX time dry weight (2.66 g) compared to single applications of TH (0.8 g) and KD (0.68 g). "The formulations, containing TH spore and the fungicide @ 20 ppm, would be integrated with crop managements”.
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Altinok, H. H. and O. Erdoğan. 2015. Determination of the in vitro effect of Trichoderma harzianum on phytopathogenic strains of Fusarium oxysporum. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43: 494–500.
Amini, J. 2009. Physiological race of Fusarium oxysporum f. sp. lycopersici in Kurdistan province of Iran and reaction of some tomato cultivars to race 1 of pathogen. Plant Pathology Journal, 8: 68–73.
Błaszczyk, L., M. Siwulski, K. Sobieralski, J. Lisiecka, and M. Jedryczka. 2014. Trichoderma spp. application and prospects for use in organic farming and industry. Journal of Plant Protection Research, 54(4): 309–317.
Carvalho, D. D. C., M. Lobo Junior, I. Martins, P. W. Inglis, and S. C. M. Mello. 2014. Biological control of fusarium oxysporum f. sp. phaseoli by trichoderma harzianum and its use for common bean seed treatment. Tropical Plant Pathology, 39: 384–391.
Chand, T. and C. Logan. 1984. Antagonists and parasites of Rhizoctonia solani and their efficacy in reducing stem canker of potato under controlled conditions. Transactions of the British Mycological Society, 83: 107–112.
Chaparro, A. P., L. H. Carvajal, and S. Orduz. 2011. Fungicide tolerance of Trichoderma asperelloides</i> and <i>T. harzianum strains</i> Agricultural Science Journal, 2: 301–307.
Chung, W. H., W. C. Chung, P. F. Ting, C. C. Ru, H. C. Huang, and J. W. Huang. 2009. Nature of resistance to methyl benzimidazole carbamate fungicides in Fusarium oxysporum f.sp. lilii and F. oxysporum f.sp. gladioli in Taiwan. Journal of Phytopathology, 157: 742–747.
Erayya, N., S. A. A Shukla, K. Tewari and J. Kumar. 2020. Compatibility of Trichoderma spp. with copperchitosan graded combinations . Plant Disease Research, 35: 42.
Fischer, B. M., I. Salakhutdinov, M. Akkurt, R. Eibach, K. J. Edwards, R. Töpfer, and E. M. Zyprian. 2004. Quantitative trait locus analysis of fungal disease resistance factors on a molecular map of grapevine. Theoretical and Applied Genetics, 108: 501–515.
Goodall, D. W. 1945. The distribution of weight change in the young tomato plant. Annals of Botany, 9: 101–103.
Haugland, E. and L. O. Brandsaeter. 1996. Experiments on bioassay sensitivity in the study of allelopathy. Journal of Chemical Ecology, 22: 1845–1859.
Jamil, A. 2021. Antifungal and plant growth promoting activity of Trichoderma spp. Against Fusarium oxysporum f. sp. lycopersici colonizing tomato. Journal of Plant Protection Research, 61: 243–253.
Kredics, L., L. Hatvani, Z. Antal, A. Szekeres, L. Manczinger, and E. Nagy Lászlóné Antal. 2004. Protease over-production in the presence of copper by a Trichoderma harzianum strain with biocontrol potential. arthropod pests by BCAs IOBC/wprs Bull. Management of Plant Diseases, 27: 373–374.
Ladi, E., N. Shukla, Y. Bohra, A. K. Tiwari, and J. Kumar. 2020. Copper tolerant Trichoderma asperellum increases bio-efficacy of copper against Phytophthora infestans in dual combination. Phytoparasitica, 48: 357–370.
Lan, W. J., Y. Zhao, Z. L. Xie, and others. 2012. Novel sorbicillin analogues from the Marine fungus Trichoderma sp. associated with the seastar Acanthaster planci. Natural Product Communications, 7: 1337–1340.
Lavanya, S. N., S. Niranjan-Raj, S. C. Nayaka, and K. N. Amruthesh. 2017. Systemic protection against pearl millet downy mildew disease induced by cell wall glucan elicitors from Trichoderma hamatum UOM 13. Journal of Plant Protection and Research, 57: 296–308.
Liu, S., L. Fu, S. Wang, J. Chen, J. Jiang, Z. Che, Y. Tian, and G. Chen. 2021. Carbendazim Resistance of Fusarium graminearum from Henan Wheat. Plant Disease, 103: 2536–2540.
Markey, B., F. Leonard, M. Archambault, A. Cullinane, and D. Maguire. 2013. Clinical veterinary microbiology e-book, Elsevier Health Sciences. 222-229
Mishra, P. K., A. N. Mukhopadhyay and U. S. Singh. 2004. Suppression of Fusarium oxysporum f. sp. gladioli populations in soil by application of Trichoderma virens and in vitro approaches for understanding biological control mechanisms. Indian Phytopathology, 57: 44–47.
Molnar, A., M. Pesti, and L. Hornok. 1985. Isolation, regeneration, and fusion of Fusarium oxysporum protoplasts. Acta Phytopathology Academiae Scientiarum Hungaricae, 20: 175–182.
Neely, D. and C. H. Beckman. 1990. The Nature of Wilt Diseases in Plants. Mycologia, 82: 668.
Poveda, J. 2021. Trichoderma as biocontrol agent against pests: New uses for a mycoparasite. Biological Control, 159: 12–26.
Royse, D. J. 1978. The Influence of Fungi Isolated from Peach Twigs on the Pathogenicity of Cytospora cincta. Phytopathology, 68: 603.
Schmitz, H., E. E. Bateman, R. H. Colley, and others. 1930. A Suggested Toximetric Method for Wood Preservatives. Industrial and Engineering Chemistry Research, 2: 361–363.
Su, Z.., A. Bernardo, B. Tian, H. Chen, S. Ma. H. Wang, S. Cai, D. Liu, D. Zhang, T. Li and H. Trick. 2019. A deletion mutation in TaHRC confers Fhb1 resistance to Fusarium head blight in wheat. Nature Genetics, 51(7): 1099-1105.
Srivastava, M., and M. Shahid. 2014. Trichoderma Genome to Genomics: A Review. J. Data Mining Genomics Proteomics, 05: 12–26.
Valarmathi, P. 2013. Compatibility of Copper hydroxide (Kocide 3000) with Biocontrol Agents. IOSR Journal of Agriculture and Veternary Sciences, 3: 28–31.
VI, G. 2018. Evaluation of Antagonistic Effect of Trichoderma Harzianum against Fusarium oxysporum causal Agent of White Yam (Dioscorearotundata poir) Tuber Rot. A Trend in Technical and Scientific Research, 1: 12–26.
Wall, M. T., D. C. McGee, and J. S. Burris. 1983. Emergence and Yield of Fungicide‐Treated Soybean Seed Differing in Quality 1. Agronomy Journal, 75: 969–973.
Weindling, R. 1934. Studies on a lethal principle effective in the parasitic action of Trichoderma lignorum on Rhizoctonia solani and other soil fungi. Phytopathology, 24: 1153–1179.
DOI: https://doi.org/10.33866/phytopathol.034.01.0755
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