SURVEY OF BARLEY STRIPE MOSAIC VIRUS (BSMV) IN MULTIPLE CEREAL VARIETIES CULTIVATED IN CHLEF PROVINCE, NORTHWEST OF ALGERIA

Abstract

This study was designed to survey Barley Stripe Mosaic Virus (BSMV) in various cereal varieties cultivated in 28 fields cultivated in seven districts namely Boukadir, Sobha, Ouled Fares, Tadjena, Kaloul, Abou Elhacene and El Karimia located in Chlef province, North West of Algeria. The survey covered 2 barley, 2 durum wheat, 4 common wheat and one oat varieties and conducted from February to the May 2017. The main objectives of this investigation were to determine the incidence of Barley Stripe Mosaic Virus in crop cereals cultivated in Algeria as well as to verify the resistant varieties. The Barley Stripe Mosaic Virus was examined in plants and seeds symptomatically by mechanical inoculation and serologically by TAS-ELISA technique using specific monoclonal antibodies. The symptoms surveying noticed more expression in spike cereals. The results of serological analysis revealed infection of 27 fields that present 96.24%. The mechanical inoculation test proved infection of totality of seed lots and seeds transmission test showed efficiency of seed transmission with a rate of 77%. In terms of species susceptibility, barley was the most susceptible crop followed by durum wheat and common wheat, whereas the oat was not infected. This investigation is the first report on Barley Stripe Mosaic Virus naturally infecting cereals in the cereal producer zone of Chlef. The findings provide useful information on viral infection of cereal fields, thus, as a priority measurement there is a need to reinforce control of cereal seeds.