Email this article 
Hide
Show all
ANTIMICROBIAL AND PLANT-GROWTH PROMOTING PROPERTIES OF RHIZOBACTERIA ISOLATED FROM WEED PLANT SENNA OCCIDENTALIS
Abstract
The use of microorganisms to increase productivity and maintain soil fertility is an alternative sustainable agricultural practice to replace agrochemicals. The purpose of this study was to isolate microorganisms with high-antifungal, -antibacterial and -plant growth promoting activity from the rhizosphere of the weed Senna occidentalis native to West Africa. Out of 14 isolates, 5 inhibited the growth of Fusarium oxysporum f. sp. radicis lycopersici (Forl) ZUM2407 and one inhibited the growth of Curtobacterium flaccumfaciens. Based on the 16S rRNA gene sequence analysis, the isolates were identified as Streptomyces hydrogenans KS13AU, Bacillus megaterium KSO8AU, B. subtilis KS07AU, B. subtilis KS11AU, and B. velezensis KS04AU. The plant growth-promoting ability of the isolated bacteria as a single-strain and consortium inoculant was tested on spring wheat (Triticum aestivum). The isolate inoculants were applied on seeds as vegetative cells. The results showed that both forms of inoculation had a positive effect on spring wheat growth. However, the greatest effect on the spring wheat plant was observed when isolates were inoculated as a consortium. The biometric parameters of the plant, such as dry biomass, shoot length, and root length, increased significantly up to 30% compared to single strain inoculations.
Keywords
Full Text:
PDFReferences
Ahkami, A.H., R. A. White III, P.P. Handakumbura and C. Jansson. 2017. Rhizosphere engineering: enhancing sustainable plant ecosystem productivity. Rhizosphere, 3: 233-243.
Arias, M. M., L. F. Leandro and G.P. Munkvold. 2013. Aggressiveness of Fusarium species and impact of root infection on growth and yield of soybeans. Phytopathology, 103, 822-832.
Arun, B., B. Gopinath and S. Sharma. 2012. Plant growth promoting potential of bacteria isolated on N free media from rhizosphere of Cassia occidentalis. World Journal of Microbiology and Biotechnology, 28: 2849-2857.
Awika, J.M. 2011. Major cereal grains production and use around the world. In Advances in cereal science: implications to food processing and health promotion. American Chemical Society, 1089: 1-13.
Bradáčová, K., A. S. Florea, A. Bar-Tal, D. Minz, U. Yermiyahu, R. Shawahna, J. Kraut-Cohen, A. Zolti, R. Erel, K. Dietel and M. Weinmann. 2019a. Microbial consortia versus single-strain inoculants: an advantage in PGPM-assisted tomato production?. Agronomy, 9: 105-111.
Bradáčová, K., M. Sittinger, K. Tietz, B. Neuhäuser, E. Kandeler, N. Berger, U. Ludewig and G. Neumann. 2019b. Maize inoculation with microbial consortia: contrasting effects on rhizosphere activities, nutrient acquisition and early growth in different soils. Microorganisms, 7: 329-335.
Cao, Y., H. Pi, P. Chandrangsu, Y. Li, Y. Wang, H. Zhou, H. Xiong, J. D. Helmann and Y. Cai. 2018. Antagonism of two plant-growth promoting Bacillus velezensis isolates against Ralstonia solanacearum and Fusarium oxysporum. Scientific reports, 8: 1-4.
Chamkhi, I., N. El Omari, A. Balahbib, N. El Menyiy, T. Benali and C. Ghoulam. 2021. Is the rhizosphere a source of applicable multi-beneficial microorganisms for plant enhancement?. Saudi Journal of Biological Sciences, 29: 1246-1259.
Chaparro, J. M., D. V. Badri, M. G. Bakker, A. Sugiyama, D. K. Manter and J. M. Vivanco. 2013. Root exudation of phytochemicals in Arabidopsis follows specific patterns that are developmentally programmed and correlate with soil microbial functions. PloS one, 8: e55731.
Chen, G., M. Khojasteh, A. Taheri-Dehkordi, S. M. Taghavi, Rahimi and E. Osdaghi. 2021. Complete genome sequencing provides novel insight into the virulence repertories and phylogenetic position of dry beans pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens. Phytopathology, 111: 268-280.
D Miljaković, D., J. Marinković, and S. Balešević-Tubić. 2020. The significance of Bacillus spp. in disease suppression and growth promotion of field and vegetable crops. Microorganisms, 8: 1037-1043.
Da Silva, I. F. and E. A. Vieira. 2019. Phytotoxic potential of Senna occidentalis (L.) Link extracts on seed germination and oxidative stress of Ipê seedlings. Plant Biology, 21: 770-779.
Damalas, C.A. and I. G. Eleftherohorinos. 2011. Pesticide exposure, safety issues, and risk assessment indicators. International Journal of Environmental Research, 8: 1402-1019.
EPPO. 2019. EPPO Standard PM 1/2(28). EPPO A1 and A2 Lists of Pests Recommended for Regulation as Quarantine Pests.
Gao, Z, B. Zhang, H. Liu, J. Han and Y Zhang. 2017. Identification of endophytic Bacillus velezensis ZSY-1 strain and antifungal activity of its volatile compounds against Alternaria solani and Botrytis cinerea. Biological Control, 105: 27-39.
Gilchrist, L. and H. J. Dubin. 2002. Fusarium head blight. FAO Plant Production and Protection Series (FAO).
Gill, H. K. and H. Garg. 2014. Pesticide: environmental impacts and management strategies. Pesticides-toxic aspects, 8: 187-195.
Guetsky R., D. Shtienberg, Y. Elad, E. Fischer and A. Dinoor. 2002. Improving biological control by combining biocontrol agents each with several mechanisms of disease suppression. Phytopathology, 92: 976-985.
Hardoim, P.R., L. S. Van Overbeek, G. Berg, A. M. Pirttilä, S. Compant, A. Campisano, M. Döring and A. Sessitsch. 2015. The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial endophytes. Microbiology and Molecular Biology Reviews, 79: 293-320.
Hayat, R., S. Ali, U. Amara, R. Khalid and I. Ahmed. 2010. Soil beneficial bacteria and their role in plant growth promotion: a review. Annals of microbiology, 60: 579-598.
Hussain, H. S. and Y.Y. Deeni. 1991. Plants in Kano ethnomedicine; screening for antimicrobial activity and alkaloids. International Journal of Pharmacognosy and Phytochemical Research, 29: 51-56.
Jeger M, C. Bragard, D. Caffier, T. Candresse, E. Chatzivassiliou, K. Dehnen‐Schmutz, G. Gilioli, J. C. Grégoire, J. A. J. Miret and A. MacLeod. 2018. Pest categorisation of Curtobacterium flaccumfaciens pv. flaccumfaciens. EFSA Journal, 16: e05299.
Panke-Buisse, K., A.C. Poole, J. K. Goodrich, R. E. Ley and J. Kao-Kniffin. 2015. Selection on soil microbiomes reveals reproducible impacts on plant function. The Multidisciplinary Journal of Microbial Ecology, 9: 980-989.
Kaur, T., A. Vasudev, S. K. Sohal and R. K. Manhas. 2014. Insecticidal and growth inhibitory potential of Streptomyces hydrogenans DH16 on major pest of India, Spodoptera litura (Fab.) (Lepidoptera: Noctuidae). BMC microbiology, 1:1-9.
Kaur, T. and R. K. Manhas. 2014. Antifungal, insecticidal, and plant growth promoting potential of Streptomyces hydrogenans DH16. Journal of Basic Microbiology, 54: 1175-1185.
Keller, M.D., G.C. Bergstrom and E.J. Shields. 2014. The aerobiology of Fusarium graminearum. Aerobiologia, 30: 123-136.
Lane, D. J., B. Pace, G. J. Olsen, D. A. Stahl, M. L. Sogin and N. R. Pace.1985. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. The Proceedings of the National Academy of Sciences, 82: 6955-6959.
Lugtenberg, B. 2015. Life of microbes in the rhizosphere. In Principles of plant-microbe interactions. Springer, Cham, 7-15.
Maksimov, I. V., R. R. Abizgil’Dina and L. I. Pusenkova. 2011. Plant growth promoting rhizobacteria as alternative to chemical crop protectors from pathogens. Applied Biochemistry and Microbiology, 47: 333-345.
Munif, A., E. Butarbutar, A. P. Pradana and A. I. Yousif. 2022. Plant-parasitic nematodes associated with common horticultural weeds. Pakistan Journal of Phytopathology, 34: 01-11.
Nelson, P. E., T. A. Toussoun and W. F. O. Marasas. 1983. Fusarium Species: An Illustrated Manual for Identification. Pennsylvania State University Press; University Park, PA, USA.
Niu, B., W. Wang, Z. Yuan, R. R. Sederoff, H. Sederoff, V. L. Chiang and R. Borriss. 2020. Microbial interactions within multiple-strain biological control agents impact soil-borne plant disease. Frontiers in Microbiology, 2452.
Oerke, E. C. 2006. Crop losses to pests. The Journal of Agricultural Science, 144: 31-43.
Palazzini, J. M., C. A. Dunlap, M. J. Bowman, and S.N. Chulze. 2016. Bacillus velezensis RC 218 as a biocontrol agent to reduce Fusarium head blight and deoxynivalenol accumulation: Genome sequencing and secondary metabolite cluster profiles. Microbiological research, 192: 30-36.
Phi, Q.T., T.T. Nguyen, M. V. Dinh, B. N. Pham and Q.T. Do. 2021. Antagonistic activity of endophytic bacteria isolated from weed plant against stem end rot pathogen of pitaya in Vietnam. Egyptian Journal of Biological Pest Control, 31:1-8.
Raaijmakers, J. M. and B. J. Lugtenberg. 2013. Perspectives for rhizosphere research. Molecular microbial ecology of the rhizosphere, 1:1227-1232.
Santoyo, G., P. Guzmán-Guzmán, F. I. Parra-Cota, S. D. Santos-Villalobos, M. Orozco-Mosqueda and B. R. Glick. 2021. Plant growth stimulation by microbial consortia. Agronomy, 11: 219-225.
Shafi, J., H. Tian and M. Ji. 2017. Bacillus species as versatile weapons for plant pathogens: a review. Biotechnology and Biotechnological Equipment. 31: 446-459.
Simons, M., A. J. Van Der Bij, I. Brand, L. A. De Weger, C. A. Wijffelman and B. J. Lugtenberg. 1996. Gnotobiotic system for studying rhizosphere colonization by plant growth-promoting Pseudomonas bacteria. Molecular plant-microbe interactions, 9: 600-607.
Sturz, A. V., B. G Matheson, W. Arsenault, J. Kimpinski and B. R. Christie. 2001. Weeds as a source of plant growth promoting rhizobacteria in agricultural soils. Canadian Journal of Microbiology, 47: 1013-1024.
Taylor, P. and R. Reeder. 2020. Antibiotic use on crops in low and middle-income countries based on recommendations made by agricultural advisors. CABI Agriculture and Bioscience, 1: 1-14.
Thomloudi, E.E., P.C. Tsalgatidou, D. Douka, T.N. Spantidos, M. Dimou, A. Venieraki and P. Katinakis. 2019. Multistrain versus single-strain plant growth promoting microbial inoculants–The compatibility issue. Hellenic Plant Protection Journal, 12: 61-77.
Usha, K., G. M. Kasturi and P. Hemalatha. 2007. Hepatoprotective effect of Hygrophila spinosa and Cassia occidentalis on carbon tetrachloride induced liver damage in experimental rats. Indian Journal of Clinical Biochemistry, 2: 132-135.
Viaene, T., S. Langendries, S. Beirinckx, M. Maes and S. Goormachtig. 2017. Streptomyces as a plant's best friend?. FEMS microbiology ecology, 92: 1-12.
Wang, H., H. Yan, J. Shin, L. Huang, H. Zhang and W. Qi. 2011. Activity against plant pathogenic fungi of Lactobacillus plantarum IMAU10014 isolated from Xinjiang koumiss in China. Annals of Microbiology, 61: 879-885.
DOI: https://doi.org/10.33866/phytopathol.034.02.0783
Refbacks
- There are currently no refbacks.
Copyright (c) 2022 Roderic Gilles Claret Diabankana, Shamil Validov, Daniel Mawuena Afordoanyi
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
 |  |  | ||
| Pakistan Journal of Phytopathology ISSN: 1019-763X (Print), 2305-0284 (Online). © 2013 Pak. J. Phytopathol. All rights reserved. | ||||