Email this article 
Hide
Show all
ANTIFUNGAL ACTIVITY OF CITRUS AURANTIUM L. ESSENTIAL OIL AGAINST CROWN ROT OF WHEAT CAUSED BY FUSARIUM GRAMINEARUM
Abstract
Fusarium graminearum a causal agent of crown rot of wheat is an important soil-borne pathogen that causes yield reduction on cereal crops. Controlling this disease is crucial to ensure food security. The present study was conducted toward the research of biological method against this pathogen. Our research aimed to evaluate the antifungal activity of Citrus aurantium essential oil (EO) at different concentrations against F. graminearum in vitro. Also, we assessed the inhibitory effect of EO on disease development in greenhouse conditions. The results showed that EO exhibited a potent antifungal activity with no significant difference compared to chemical fungicide, Difenoconazole. The tested EO effectively inhibited radial growth of the pathogen in vitro, and it revealed significant decreasing on disease incidence from 91.7% to 32.8% in the in vivo bioassay. In addition, the seed treatment with C. aurantium EO improved significantly the growth parameters of the wheat seedlings compared to the untreated control. The chemical characterization of C. aurantium essential oil using the gas chromatography analysis allowed the identification of monoterpene hydrocarbon as major group of volatile compounds. These were Limonene (17.6%), Geranyl acetate (11.9%), Geranial (10.8%), Citral (10.3%), Linalool (9.9%) and Linalyl acetate (5.7%). From the current study, we can deduce that essential oils extracted from C. aurantium L. leaves can be used as an antifungal bio-product against phytopathogenic Fusarium species.
Keywords
Full Text:
PDFReferences
Abdallah-Nekache, N., I. Laraba, C. Ducos, C. Barreau, Z. Bouznad and H. Boureghda. 2019. Occurrence of Fusarium head blight and Fusarium crown rot in Algerian wheat: identification of associated species and assessment of aggressiveness. European Journal of Plant Pathology, 154: 499-512.
Ali, A., M. A. Abro, R. N. Syed, M. Khan and H. Saeed. 2018. Effect of some essential oils on the growth and development of fruit rot of lemon caused by Aspergillus niger under in-vitro conditions. Pakistan Journal of Phytopathology, 30: 199-205.
Badawy, M. E., G. I. K. Marei, E. I. Rabea and N. E. Taktak. 2019. Antimicrobial and antioxidant activities of hydrocarbon and oxygenated monoterpenes against some foodborne pathogens through in vitro and in silico studies. Pesticide biochemistry and physiology, 158: 185-200.
Beccari, G., L. Covarelli and P. Nicholson. 2011. Infection processes and soft wheat response to root rot and crown rot caused by Fusarium culmorum. Plant Pathology, 60: 671-684.
Bnina, E.B., H. Hajlaoui, I. Chaieb, M. Daami-Remadi, M. Ben Said and H. Ben Jannet. 2019. Chemical composition, antimicrobial and insecticidal activities of the Tunisian Citrus aurantium essential oils. Czech Journal of Food Sciences, 37: 81–92.
Brand-Williams, W., M.E. Cuvelier and C. Berset. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 28: 25-30.
Chambers, J. E., H. Greim, R. J. Kendall, H. Segner, R. M. Sharpe and G. Van Der Kraak. 2014. Human and ecological risk assessment of a crop protection chemical: a case study with the azole fungicide epoxiconazole. Critical reviews in toxicology, 44: 176-210.
Chen, H.Y., Y. C. Lin and C. L. Hsieh. 2007. Evaluation of antioxidant activity of aqueous extract of some selected nutraceutical herbs. Food chemistry, 104: 1418-1424.
Değirmenci, H. and H. Erkurt. 2020. Relationship between volatile components, antimicrobial and antioxidant properties of the essential oil, hydrosol and extracts of Citrus aurantium L. flowers. Journal of Infection and Public Health, 13(1): 58-67.
Demirci, E. and E. Dane. 2003. Identification and pathogenicity of Fusarium spp. from stem bases of winter wheat in Erzurum, Turkey. Phytoparasitica, 31: 170-173.
Dosoky, N. S. and W. N. Setzer. 2018. Biological activities and safety of Citrus spp. essential oils. International journal of molecular sciences, 19: 1966.
Elgat, W. A., A. M. Kordy, M. Böhm, R. Černý, A. Abdel-Megeed and M. Z. Salem. 2020. Eucalyptus camaldulensis, Citrus aurantium, and Citrus sinensis Essential Oils as Antifungal Activity against Aspergillus flavus, Aspergillus niger, Aspergillus terreus, and Fusarium culmorum. Processes, 8: 1003.
Elgayyar, M., F. A. Draughon, D. A. Golden and J. R. Mount. 2001. Antimicrobial activity of essential oils from plants against selected pathogenic and saprophytic microorganisms. Journal of food protection, 64: 1019-1024.
Ellouze, I., M. Abderrabba, N. Sabaou, F. Mathieu, A. Lebrihi and J. Bouajila. 2012. Season's variation impact on Citrus aurantium leaves essential oil: chemical composition and biological activities. Journal of food science, 77: T173-T180.
Gaff, M., M. Esteban-Decloux and P. Giampaoli. 2020. Bitter orange peel essential oil: a review of the different factors and chemical reactions influencing its composition. Flavour and Fragrance Journal, 35: 247-269.
Grey, W. and D. Mathre. 1984. Reaction of spring barleys to common root rot and its effect on yield components. Canadian Journal of Plant Science, 64: 245-253.
Hahn, M. 2014. The rising threat of fungicide resistance in plant pathogenic fungi: Botrytis as a case study. Journal of chemical biology, 7: 133-141.
Hajlaoui, H., S. Arraouadi, K. Aouadi, M. Snoussi, E. Noumi and A. Kadri. 2021. GC-MS profile, α-glucosidase inhibition potential, antibacterial and antioxidant evaluation of peels Citrus aurantium L., essential oil. Journal of Pharmaceutical Research International, 33: 1580-1591.
Jiang, M. H., L. Yang, L. Zhu, J. H. Piao and J. G. Jiang. 2011.Comparative GC/MS analysis of essential oils extracted by 3 methods from the bud of Citrus aurantium L. var. amara Engl. Journal of food science, 76: C1219-C1225.
Jing, L., Z. Lei, L. Li, R. Xie, W. Xi, Y. Guan, L. Sumner and Z. Zhou. 2014. Antifungal activity of Citrus essential oils. Journal of agricultural and food chemistry, 62: 3011-3033.
Kim, K.H., E. Kabir and S. A. Jahan. 2017. Exposure to pesticides and the associated human health effects. Science of the total environment, 575: 525-535.
Konuk, H. B. and B. Ergüden. 2020. Phenolic–OH group is crucial for the antifungal activity of terpenoids via disruption of cell membrane integrity. Folia Microbiologica, 65: 775-783.
Lang, G. and G. Buchbauer. 2012. A review on recent research results (2008–2010) on essential oils as antimicrobials and antifungals. A review. Flavour and Fragrance Journal, 27: 13-39.
Mejri, H., T. Khetatfa, W. Aidi Wannes, A. Smaoui and M. Saidani Tounsi. 2022. Histochemistry, chemical composition and antioxidant activity of Citrus aurantium L. essential oil during leaf development. Journal of Essential Oil Research, 34: 329-338.
Moraes, T.M., H. Kushima, F.C. Moleiro, R.C. Santos, L.R. Machado Rocha, M.O. Marques, W. Vilegas and C.A. Hiruma-Lima. 2009. Effects of limonene and essential oil from (Citrus aurantium) on gastric mucosa: Role of prostaglandins and gastric mucus secretion. Chemico-Biological Interactions, 180: 499–505.
Moutassem, D., L. Belabid, Y. Bellik, S. Ziouche and F. Baali. 2019. Efficacy of essential oils of various aromatic plants in the biocontrol of Fusarium wilt and inducing systemic resistance in chickpea seedlings. Plant Protection Science, 55: 202-217.
O’Mara, S. P., K. Broz, M. Boenisch, Z. Zhong, Y. Dong and H. C. Kistler. 2020. The Fusarium graminearum t-SNARE Sso2 is involved in growth, defense, and DON accumulation and virulence. Molecular Plant-Microbe Interactions, 33: 888-901.
Ortuño, A., L. Díaz, N. Alvarez, I. Porras, A. García-Lidón and J. Del Río. 2011. Comparative study of flavonoid and scoparone accumulation in different Citrus species and their susceptibility to Penicillium digitatum. Food chemistry, 125: 232-239.
Ouedrhiria, W., S. Bouhdida, M. Balouiria, A. El Ouali Lalamib, S. Mojac Ouazzani, F. Chahdid and H. Greche. 2015. Chemical composition of Citrus aurantium L. leaves and zest essential oils, their antioxidant, antibacterial single and combined effects. Journal of Chemical and Pharmaceutical Research, 7: 78-84.
Qin, J., M. Wu and S. Zhou. 2020. FgEaf6 regulates virulence, asexual/sexual development and conidial septation in Fusarium graminearum. Current Genetics, 66: 517-529.
Reyes-Jurado, F., A. R. Navarro-Cruz, C. E., Ochoa-Velasco, E. Palou, A. López-Malo and R. Ávila-Sosa. 2020. Essential oils in vapor phase as alternative antimicrobials: A review. Critical reviews in food science and nutrition, 60: 1641-1650.
Ruberto, G. and M. T. Baratta. 2000. Antioxidant activity of selected essential oil components in two lipid model systems. Food chemistry, 69: 167-174.
Sarrou, E., P. Chatzopoulou, K. Dimassi-Theriou and I. Therios. 2013. Volatile constituents and antioxidant activity of peel, flowers and leaf oils of Citrus aurantium L. growing in Greece. Molecules, 18: 10639-10647.
Soylu, E., S. Soylu and S. Kurt. 2006. Antimicrobial activities of the essential oils of various plants against tomato late blight disease agent Phytophthora infestans. Mycopathologia, 161: 119-128.
Trabelsi, D., A. M. Hamdane, M. B. Said and M. Abderraba. 2016. Chemical composition and antifungal activity of essential oils from flowers, leaves and peels of Tunisian Citrus aurantium against Penicillium digitatum and Penicillium italicum. Journal of Essential Oil Bearing Plants, 19: 1660-1674.
Tunali, B., J. M. Nicol, Z. Hodson, O. Uçkun, D. Büyük and D. Erdurmuş. 2008. Root and crown rot fungi associated with spring, facultative, and winter wheat in Turkey. Plant Disease, 92: 1299-1306.
Vaou, N., E. Stavropoulou, C. Voidarou, C. Tsigalou and E. Bezirtzoglou. 2021. Towards advances in medicinal plant antimicrobial activity: A review study on challenges and future perspectives. Microorganisms, 9: 2041.
Wan, J., S. Zhong, P. Schwarz, B. Chen and J. Rao. 2019. Physical properties, antifungal and mycotoxin inhibitory activities of five essential oil nanoemulsions: Impact of oil compositions and processing parameters. Food chemistry, 291: 199-206.
Yadav, R. and M. Agarwala. 2011. Phytochemical analysis of some medicinal plants. Journal of phytology, 3: 10-14.
Yildiz, E., M. Kaplankiran, T. H. Demirkeser and C. Toplu. 2013. Seasonal Patterns of Carbohydrates in Mandarin cvs.'Fremont','Nova'and'Robinson'on Different Rootstocks. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41: 255-262.
Yu, L., M. Chen, J. Liu, X. Huang, W. He, Z. Qing and J. Zeng. 2020. Systematic detection and identification of bioactive ingredients from Citrus aurantium L. var. amara using HPLC-Q-TOF-MS combined with a screening method. Molecules, 25: 357-362.
Zabka, M. and R. Pavela. 2018. Review Chapter: Fusarium genus and essential oils in: Natural Antimicrobial Agents. (ed.). Springer. P. 95-120
DOI: https://doi.org/10.33866/phytopathol.034.02.0798
Refbacks
- There are currently no refbacks.
Copyright (c) 2022 Kamel eddine Makhlouf, karima boungab, abdelkader elouissi, zine eddine daikh
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
 |  |  | ||
| Pakistan Journal of Phytopathology ISSN: 1019-763X (Print), 2305-0284 (Online). © 2013 Pak. J. Phytopathol. All rights reserved. | ||||