SCREENING OF PHYTOCHEMICALS AND IN VITRO ANTIDIABETIC POTENTIAL OF RAPHANUS SATIVUS LEAVES EXTRACT

Abstract

Now a day, plants are gaining importance globally due to pharmacological and medicinal behavior. The current study aimed to evaluate the phytochemical screening and antidiabetic potential of Raphanus sativus leaves extract. The leaves extract of Raphanus sativus was prepared through maceration using different solvents such as n-hexane, chloroform, ethyl acetate, n-butanol, and methanol for one week. Qualitative analysis was carried out using standard protocols. Quantitative analysis was also performed for the determination of total alkaloids, saponin, total phenolic content, total flavonoid content, and antidiabetic activity. The total phenolic contents (TPC) and total flavonoid contents (TFC) were analyzed by spectroscopic technique. The alpha amylase inhibition was also done through a standard procedure cited in the literature. The preliminary phytochemical screening showed that the ethyl acetate extract extracted the maximum amount of flavonoids and alkaloids compared to other extracts. The quantitative analysis showed that the total phenolic and flavonoid contents ranged from (640.7 ± 6.4 to 154.9 ± 1.6) GAE mg/100 g and (103.6± 1.1 to 6.5 ± 0.1) per mg/100 g GAE, respectively. All of the plant extracts (Raphanus sativus) inhibited alpha amylase, but the ethyl acetate extract (36.2% inhibition) was the most potent. The findings of the studies reflect the importance of medicinal flora to counteract the adverse effects of drugs as well as the internally produced free radicals, responsible for metabolic disorders, including diabetes. So, the plant extract Raphanus sativus may be used as an herbal remedy alone as well as with drugs to cure metabolic disorders.