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EIGHT YEARS PRESERVATION, MORPHOLOGICAL STABILITY AND PURITY OF COCHLIOBOLUS SATIVUS
Abstract
While spot blotch and common root rot caused by the fungus Cochliobolus sativus are destructive diseases of wheat and barley which lead to significant yield losses globally, few publications have appeared on preservation which is important in understanding relationships of this pathogen with its host. The aim of this study was to preserve 32 isolates causing spot blotch and 22 isolates causing common root rot differing in their phenotypical characters in two different methods: the storage treatments included fungal suspension (spores and hyphae) in sterile distilled water at 4°C and fungal cultures on Petri-dishes after reaching suitable growth by freezing at -16°C. The viability, morphological alteration and contamination by other microorganisms were then verified: phenotypic characteristics were evaluated qualitatively at 6, 12, 18, 24, 30, 36, 60, 96 months. All C. sativus remained viable and the morphological alteration and contamination by bacteria or other fungi were not observed after 8 years of storage in cold water and by freezing. Neither time in storage nor isolate of two diseases was associated with a lack of viability. This study helps to retain viability and morphological stability for 55 isolates of C. sativus by the two tested storage methods for over 96 months.
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DOI: https://doi.org/10.33866/phytopathol.035.02.0965
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