THE RESISTANCE RESPONSE OF SIX RICE VARIETIES TO RICE RAGGED STUNT VIRUS IN SOUTH SULAWESI, INDONESIA: A GREENHOUSE STUDY

Saipul Abbas, Sri Sulandari, Sedyo Hartono, Jumardin Jumardin, Akhmad Rizali, Ernawati Djaya, Erwin Najamuddin, Ayyub Ar Rahman, Aminah Aminah, Hasbi Hasbi, Surianto Sipi

Abstract


Nilaparvata lugens, commonly known as the brown planthopper (BPH), transmits the Rice ragged stunt virus (RRSV) which severely affects rice plants. This study aimed to evaluate the resistance of six rice varieties to RRSV in South Sulawesi under greenhouse conditions. The stages of the study included the preparation of the inoculum source, seeding of rice seeds, rearing of BPH, transmission treatment, and observation of resistance response and symptom scoring based on IRRI (1996) in a greenhouse. Data analysis techniques using Duncan's 5% were also applied. The results showed that varieties Inpari 36, 37, TN1, Mekongga, Ciherang, and Tukad Unda exhibited differing resistance levels, with Tukad Unda showing the highest resistance. The disease incidence ranged from 63.33% to 100%, and disease intensity varied from 28.14% to 71.10%. These findings suggest that the selection of resistant varieties could significantly mitigate RRSV impact, highlighting the need for integrated pest management strategies in rice cultivation.


Keywords


Disease intensity, Plant resistance, Rice varieties, Rice ragged stunt virus, South Sulawesi

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References


Baehaki S.E., E.H. Iswanto and D. Munawar. 2016. Resistensi wereng cokelat terhadap insektisida yang beredar di sentra produksi padi. Jurnal Penelitian Pertanian Tanaman Pangan, 35(2): 124705. https://doi.org/10.21082/jpptp.v35n2.2016.p99-108

Baehaki, S.E. 2012. Perkembangan biotipe hama wereng coklat pada tanaman padi. IPTEK Tanaman Pangan, 7(1): 8-17.

Baehaki, S.E. and D. Munawar. 2008. Identifikasi biotipe wereng coklat di Jawa, Sumatera dan Sulawesi dan reaksi ketahanan kultivar padi. In Prosiding Seminar Apresiasi Hasil Penelitian Padi Menunjang P2BN. pp. 351-366).

BAO, Y.Y. and C.X. ZHANG. 2019. Recent advances in molecular biology research of a rice pest, the brown planthopper. Journal of Integrative Agriculture, 18(4): 716-728. https://doi.org/10.1016/S2095-3119(17)61888-4

Bastian A. 2005. Evaluasi Ketahanan Galur-Galur Padi Sawah terhadap Penyakit Tungro. Prosiding Seminar Ilmiah Dan Pertemuan Tahunan Komda Sul-Sel, 53–56.

Cabauatan, P.Q., R.C. Cabunagan and I.R. Choi. 2009. Rice viruses transmitted by the brown planthopper Nilaparvata lugens Stål. Planthoppers: New threats to the sustainability of intensive rice production systems in Asia. pp.357-368.

Cheng, H.N., J.C. Chen, H. His, L.M. Yang, T.L. Chu, C.T. Wu, J.K. Chien and C.S. Yang. 1979. Studies on the migrations of brown planthopper Nilaparvata lugens Stal. K'un ch'ung hsueh pao. Acta entomologica sinica.

Chomchan, P., G.J. Miranda and Y. Shirako. 2002. Detection of Rice grassy stunt tenuivirus nonstructural proteins p2, p5 and p6 from infected rice plants and from viruliferous brown planthoppers. Archives of virology, 147: 2291-2300.

Effendi, B.S. and D. Munawar. 2013. Uji ketahanan galur padi terhadap wereng coklat biotipe 3 melalui population build-up. Jurnal Entomologi Indonesia, 10(1): 7-7. https://doi.org/http://dx.doi.org/10.5994/jei.10.1.7

Helina, S., S. Sulandari, S. Hartono and A. Trisyono. 2018. Detection and transmission of rice stunt virus on Ciherang and Situ Bagendit varieties. Lampung University. https://doi.org/10.23960/j.hptt.218169-176

Hibino, H. 1977. A virus disease of rice (kerdil hampa) transmitted by the brown planthopper, Nilaparvata lugens Stal, in Indonesia. Contribution of the Central Research Institute for Agriculture, Bogor, Indonesia, 35: 1-15.

Hibino, H. 1996. Biology and epidemiology of rice viruses. Annual review of phytopathology, 34(1): 249-274. https://doi.org/10.1146/annurev.phyto.34.1.249

Hibino, H., M. Roechan and S. Sudarisman. 1978. Association of two types of virus particles with penyakit habang (tungro disease) of rice in Indonesia. Phytopathology, 68(10): 1412-1416.

Hogenhout, S.A., E.D. Ammar, A.E. Whitfield and M.G. Redinbaugh. 2008. Insect vector interactions with persistently transmitted viruses. Annual Review of Phytopathology, 46: 327-359. https://doi.org/10.1146/annurev.phyto.022508.092135

Huang, H.J., C.W. Liu, X.H. Huang, X. Zhou, J.C. Zhuo and Y.Y. Bao. 2017. Mucin-like protein, a saliva component involved in brown planthopper virulence and host adaptation. Journal of Insect Physiology, 98: 223-230. https://doi.org/10.1016/j.jinsphys.2017.01.012

Huang, H.J., C.W. Liu, X.H. Huang, X. Zhou, J.C. Zhuo, C.X. Zhang and Y.Y. Bao. 2016. Screening and functional analyses of Nilaparvata lugens salivary proteome. Journal of proteome research, 15(6): pp.1883-1896. https://doi.org/10.1021/acs.jproteome.6b00086

Huang, H.J., C.W. Liu, Y.F. Cai, M.Z. Zhang, Y.Y. Bao and C.X. Zhang. 2015b. A salivary sheath protein essential for the interaction of the brown planthopper with rice plants. Insect Biochemistry and Molecular Biology, 66: 77-87. https://doi.org/10.1016/j.ibmb.2015.10.007

Huang, H.J., Y.Y. Bao, S.H. Lao, X.H. Huang, Y.Z. Ye, J.X. Wu, H.J. Xu, X.P. Zhou and C.X. Zhang. 2015a. Rice ragged stunt virus-induced apoptosis affects virus transmission from its insect vector, the brown planthopper to the rice plant. Scientific reports, 5(1): 11413. https://doi.org/10.1038/srep11413

IRRI (International Rice Research Institute). 1996. Standard Evaluation System for Rice . Los Baños, Philippines: International Rice Research Institute.

Jena, K.K. and S.M. Kim. 2010. Current status of brown planthopper (BPH) resistance and genetics. Rice, 3(2): 161-171. https://doi.org/10.1007/s12284-010-9050-y

Jia, D., N. Guo, H. Chen, F. Akita, L. Xie, T. Omura and T. Wei. 2012. Assembly of the viroplasm by viral non-structural protein Pns10 is essential for persistent infection of Rice ragged stunt virus in its insect vector. Journal of General Virology, 93(10): 2299-2309. https://doi.org/10.1099/vir.0.042424-0

Kusuma, A.F., S. Sulandari, S. Somowiyarjo and S. Hartono. 2018. Molecular Diversity of Rice Ragged Stunt Oryzavirus in Java and Bali, Indonesia: Molecular Diversity of Rice ragged stunt virus in Indonesia. Proceedings of the Pakistan Academy of Sciences: Part B (Life and Environmental Sciences), 55(1): 57-64.

Li, S., H. Wang and G. Zhou. 2014. Synergism between Southern rice black-streaked dwarf virus and Rice ragged stunt virus enhances their insect vector acquisition. Phytopathology, 104(7): 794-799. https://doi.org/10.1094/PHYTO-11-13-0319-R

Ling, K.C. 1972. Rice virus diseases. IRRI.

Matsumura, M., H. Takeuchi, M. Satoh, S. Sanada‐Morimura, A. Otuka, T. Watanabe and D. Van Thanh. 2008. Species‐specific insecticide resistance to imidacloprid and fipronil in the rice planthoppers Nilaparvata lugens and Sogatella furcifera in East and South‐east Asia. Pest Management Science: formerly Pesticide Science, 64(11): 1115-1121. https://doi.org/10.1002/ps.1641

McPherson, M. and S. Møller. 2006. Pcr. Taylor & Francis. https://doi.org/10.1201/9780429258381

Padi B.B. 2009. Deskripsi varietas padi. Balai Penelitian Dan Pengembangan Pertanian. Departemen Pertanian, Jakarta.

Petrova, A. and C.M. Smith. 2015. Application of brown planthopper salivary gland extract to rice plants induces systemic host mRNA patterns associated with nutrient remobilization. PLoS One, 10(12): p.e0141769. https://doi.org/10.1371/journal.pone.0141769

Pham, V.D., R.C. Cabunagan, P.Q. Cabauatan, H.S. Choi, I.R. Choi, V.C. Ho and H.H. Nguyen. 2007. Yellowing syndrome of rice: etiology, current status and future challenges. Omonrice, (15): 94-101.

Saenchai, C., C. Prom-u-thai, S. Jamjod, B. Dell and B. Rerkasem. 2012. Genotypic variation in milling depression of iron and zinc concentration in rice grain. Plant and soil, 361: 271-278. https://doi.org/10.1007/s11104-012-1228-1

Sama, S., A. Hasanuddin, I. Manwan, R.C. Cabunagan and H. Hibino. 1991. Integrated management of rice tungro disease in South Sulawesi, Indonesia. Crop Protection, 10(1): 34-40. https://doi.org/10.1016/0261-2194(91)90022-J

Shimizu, T., T. Ogamino, A. Hiraguri, E. Nakazono-Nagaoka, T. Uehara-Ichiki, M. Nakajima, K. Akutsu, T. Omura and T. Sasaya. 2013. Strong resistance against Rice grassy stunt virus is induced in transgenic rice plants expressing double-stranded RNA of the viral genes for nucleocapsid or movement proteins as targets for RNA interference. Phytopathology, 103(5): 513-519. https://doi.org/10.1094/PHYTO-07-12-0165-R

Wang, H., D. Xu, L. Pu and G. Zhou. 2014. Southern rice black-streaked dwarf virus alters insect vectors' host orientation preferences to enhance spread and increase Rice ragged stunt virus coinfection. Phytopathology, 104(2): 196-201. https://doi.org/10.1094/PHYTO-08-13-0227-R

Wang, Y., Z. Song, W. Zhang, L. Xu, X. Su, E.M.M. Muleke and L. Liu. 2017. Identification and characterization of expressed TIR-and non-TIR-NBS-LRR resistance gene analogous sequences from radish (Raphanus sativus L.) de novo transcriptome. Scientia horticulturae, 216: 284-292.

Wen, Y., Z. Liu, H. Bao and Z. Han. 2009. Imidacloprid resistance and its mechanisms in field populations of brown planthopper, Nilaparvata lugens Stål in China. Pesticide Biochemistry and Physiology, 94(1): 36-42. https://doi.org/10.1016/j.pestbp.2009.02.009 BPH

Wu, J., Y. Ni, H. Liu, M. Ding and X. Zhou. 2014. Monoclonal antibody-based serological assays and immunocapture-RT-PCR for detecting Rice dwarf virus in field rice plants and leafhopper vectors. Journal of Virological Methods, 195: 134-140. https://doi.org/10.1016/j.jviromet.2013.09.013

Yang, X., K. Lv, M. Wang and G. Zhou. 2017. Investigation of viruses infecting rice in southern China using a multiplex RT-PCR assay. Crop protection, 91: 8-12.

Yangon, M. 2011. Population fluctuations of brown plant hopper Nilaparvata lugens stal. and white backed plant hopper sogatella furcifera horvath on. Journal of Entomology, 8(2): 183-190. http://dx.doi.org/10.3923/je.2011.183.190

Zadoks, J.C. and R.D. Schein. 1979. Epidemiology and plant disease management. Epidemiology and plant disease management.




DOI: https://doi.org/10.33866/phytopathol.036.01.1066

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