Email this article 
Hide
Show all
GENOTYPIC AND BIOCHEMICAL VARIATION IN THE RESPONSE OF BARLEY TO THE ROOT-KNOT NEMATODE (MELOIDOGYNE JAVANICA) AT SEEDLING STAGE
Abstract
The present study was conducted to evaluate 28 commercial cultivars and two promising breeding lines of barley in terms of resistance to Meloidogyne javanica nematode and to investigate the synthesis level of peroxidase, superoxide dismutase, catalase and polyphenol oxidase enzymes in different days after inoculation in greenhouse condition. Based on the results of evaluation, Jolge and Nimrouz cultivars were highly resistant, Rihan and Zarjow were very susceptible. The rest of the cultivars were ranked between these groups in resistant, moderately resistant and moderately susceptible groups. Comparison of the activity of antioxidant enzymes in highly resistance cultivars (Jolge and Nimrouz) and very susceptible cultivars (Rihan and Zarjow) roots showed that the activity of peroxidase, superoxide dismutase and polyphenol oxidase increased in the root of the highly resistant cultivars and decreased or remains unchanged in very susceptible cultivars. Nevertheless, catalase showed a decreasing trend after inoculation by nematodes in highly resistance cultivars, and in general; its level in resistant cultivars was less than susceptible cultivars. Therefore, changes in the activity of these enzymes can be attributed to different levels of resistance among these cultivars.
Keywords
Full Text:
PDFReferences
Abad, P., B. Favery, M.-N. Rosso and P. Castagnone-Sereno. 2003. Root-knot nematode parasitism and host response: molecular basis of a sophisticated interaction. Molecular Plant Pathology, 4: 217-224.
Aboulipour, M., M. Olia, A. Fadaee and M. Kadivar. 2011. Reaction of some cucumber cultivars to root-knot nematode, Meloidogyne javanica. Iranian Journal of Plant Pathology, 47: 279-291.
Abrahim, N. N., M. S. Kanthimathi and A. Abdul-Aziz. 2012. Piper betle shows antioxidant activities, inhibits MCF-7 cell proliferation and increases activities of catalase and superoxide dismutase. BMC Complementary and Alternative Medicine, 12.
Aebi, H. 1984. Catalase in vitro. Methods in Enzymology. Elsevier, pp. 121-126.
Aemmr, A., A. Farahat, A. Al-Sayed and N. Mahfoud. 2014. Antioxidant enzymes as well as oxidant activities involved in defense mechanisms against the root-knot, reniform and citrus nematodes in their host plants. International Journal of Biotechnology and Food Science, 2: 102-111.
Anjum, T., S. Fatima and S. Amjad. 2012. Physiological changes in wheat during development of loose smut. Tropical Plant Pathology, 37: 102-107.
Beauchamp, C. and I. Fridovich. 1971. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44: 276-287.
Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254.
Chen, Z., H. Silva and D. Klessig. 1993. Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid. Science, 262: 1883-1886.
Czembor, J. H., H. J. Czembor, G. Attene and R. Papa. 2007. Leaf rust resistance in selections from barley landraces collected in Sardinia. Plant Breeding and Seed Science, 56.
De Grisse, A. T. 1969. Redescription and modification of some techniques used in the study of nematodes phytoparasitaires. Mededelingen Rijksfacultiet Landbouw Wetenschappe Gent, 34: 351-369.
Dhandaydham, M., L. Charles, H. Zhu, J. L. Starr, T. Huguet, D. R. Cook, J.-M. Prosperi and C. Opperman. 2008. Characterization of root-knot nematode resistance in Medicago truncatula. Journal of nematology, 40: 46.
Dixit, A., G. Das, P. Dixit, A. Singh, N. Kumbhakar, M. Sankar and R. Sharma. 2017. An assessment of benzimidazole resistance against caprine nematodes in Central India. Tropical animal health and production, 49: 1471-1478.
EL-Mesalamy, A. F. M. 2013. Resistance of some plant cultivars wheat, barley and broad bean to root-knot nematode, Meloidogyne Javanica. EJAN, 12: 130-138.
Esterbauer, H., E. Schwarzl and M. Hayn. 1977. A rapid assay for catechol oxidase and laccase using 2-nitro-5-thiobenzoic acid. Analytical Biochemistry, 77: 486-494.
Ghaderi, R., L. Kashi and A. Karegar. 2014. Contribution to the study of the genus Paratylenchus Micoletzky, 1922 sensu lato (Nematoda: Tylenchulidae). Zootaxa, 3841: 151.
Guida, G., G. Zacheo and T. Bleve-Zacheo. 1992. Activation of detoxifying enzymes in tomato roots following paraquat treatment and nematode infection. Nematologia Mediterranea, 20: 203-209.
Harris, K. F. and K. Maramorosch. 2013. Pathogens, vectors, and plant diseases: approaches to control. Elsevier.
Hartman, K. and J. Sasser. 1985. Identification of Meloidogyne species on the basis of differential host test and perineal-pattern morphology.
Holterman, M., K. Rybarczyk, S. Van Den Elsen, H. Van Megen, P. Mooyman, R. P. Santiago, T. O. M. Bongers, J. Bakker and J. Helder. 2008. A ribosomal DNA-based framework for the detection and quantification of stress-sensitive nematode families in terrestrial habitats. Molecular Ecology Resources, 8: 23-34.
Hussain, M. A., T. Mukhtar and M. Z. Kayani. 2016. Reproduction of Meloidogyne incognita on resistant and susceptible okra cultivars. Pakistan Journal of Agricultural Sciences, 53: 371-375.
Hussey, R. 1973. A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Dis. Rep., 57: 1025-1028.
Ibrahim, I., M. Rezk and A. Ibrahim. 1988. Resistance of barley and wheat cultivars to root-knot nematodes, Meloidogyne spp. Pakistan Journal Nematology, 6: 39-43.
Jenkins, W. 1964. A rapid centrifugal-flotation technique for separating nematodes from soil. Plant disease reporter, 48.
Jepson, S. B. 1987. Identification of root-knot nematodes (Meloidogyne species).
Johnson, A. and R. Motsinger. 1989. Suitability of small grains as hosts of Meloidogyne species. Journal of Nematology, 21: 650.
Kaloshian, I., P. Roberts and I. Thomason. 1989. Resistance to Meloidogyne spp. in allohexaploid wheat derived from Triticum turgidum and Aegilops squarrosa. Journal of nematology, 21: 42.
Kar, M. and D. Mishra. 1976. Catalase, Peroxidase, and Polyphenoloxidase Activities during Rice Leaf Senescence. Plant Physiology, 57: 315-319.
Karajeh, M., A. Abdel-Ghani and N. Al-Majali. 2011. Response of wheat, barley and oat cultivars and accessions to Meloidogyne javanica. Nematologia Mediterranea, 39.
Karuri, H. W., D. Olago, R. Neilson, E. Mararo and J. Villinger. 2017. A survey of root knot nematodes and resistance to Meloidogyne incognita in sweet potato varieties from Kenyan fields. Crop Protection, 92: 114-121.
Kayani, M. Z. and T. Mukhtar. 2018. Reproductivity of Meloidogyne incognita on Fifteen Cucumber Cultivars. Pakistan Journal of Zoology, 50.
Kayani, M. Z., T. Mukhtar and M. A. Hussain. 2018. Interaction between Nematode Inoculum Density and Plant Age on Growth and Yield of Cucumber and Reproduction of Meloidogyne incognita. Pakistan Journal of Zoology, 50.
Kumar, A., P. Mali and V. Manga. 2010. Changes of some phenolic compounds and enzyme activities on infected pearl millet caused by Sclerospora graminicola. International Journal of plant physiology and biochemistry, 2: 6-10.
Kuvalekar, A., A. Redkar, K. Gandhe and A. Harsulkar. 2011. Peroxidase and polyphenol oxidase activities in compatible host–pathogen interaction in Jasminum officinale and Uromyces hobsoni: Insights into susceptibility of host. New Zealand Journal of Botany, 49: 351-359.
Mahdy, M. E. and M. Sally A. 2011. Physiological response of strawberry grown in root- knot nematode infested soil under different safety control applications. Arab Universities Journal of Agricultural Sciences, 19: 217-231.
Mehta, A., A. C. M. Brasileiro, D. S. L. Souza, E. Romano, M. A. Campos, M. F. Grossi-de-Sá, M. S. Silva, O. L. Franco, R. R. Fragoso, R. Bevitori and T. L. Rocha. 2008. Plant-pathogen interactions: what is proteomics telling us? FEBS Journal, 275: 3731-3746.
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 7: 405-410.
Moens, Maurice, Roland N. Perry, and James L. Starr. 2009. "Meloidogyne species–a diverse group of novel and important plant parasites." Root-knot nematodes 1 : 483.
Moghbeli, E., S. H. Nemati, H. Aroiee and J.-A. Olfati. 2017. Evaluation of resistance, enzymatic response, and phenolic compounds in roots of F1 cucumber hybrids to Fusarium oxysporum f. sp. radicis-cucumerinum. Journal of Horticultural Research, 25: 117-124.
Mohammadi, M. and H. Kazemi. 2002. Changes in peroxidase and polyphenol oxidase activities in susceptible and resistant wheat heads inoculated with Fusarium graminearum and induced resistance. Plant Science, 162: 491-498.
Mukhtar, T. 2018. Management of Root-Knot Nematode, Meloidogyne incognita, in Tomato with Two Trichoderma Species. Pakistan Journal of Zoology, 50.
Mukhtar, T., M. A. Hussain, M. Z. Kayani and M. N. Aslam. 2014. Evaluation of resistance to root-knot nematode (Meloidogyne incognita) in okra cultivars. Crop Protection, 56: 25-30.
Nayak, D. K. and R. K. Pandey. 2016. Physiological and Biochemical changes of susceptible and resistant brinjal cultivars induced by root-knot nematode, Meloidogyne incognita. Journal of Global Biosciences, 5: 4358-4368.
Ngadze, E., D. Icishahayo, T. A. Coutinho and J. E. van der Waals. 2012. Role of polyphenol oxidase, peroxidase, phenylalanine ammonia lyase, chlorogenic acid, and total soluble phenols in resistance of potatoes to soft rot. Plant Disease, 96: 186-192.
Oliveira, J. T. A., N. C. Andrade, A. S. Martins-Miranda, A. A. Soares, D. M. F. Gondim, J. H. Araújo-Filho, F. R. Freire-Filho and I. M. Vasconcelos. 2012. Differential expression of antioxidant enzymes and PR-proteins in compatible and incompatible interactions of cowpea (Vigna unguiculata) and the root-knot nematode Meloidogyne incognita. Plant Physiology and Biochemistry, 51: 145-152.
Opperman, C. H. 1988. Reproduction of Three Root-Knot Nematodes on Winter Small Grain Crops. Plant Disease, 72: 869.
Potpour, M., M. Mohammadi, M. Torabi and A. Sharifi-Tehrani. 2000. Peroxidase specific activity pattern in resistant and susceptible barley seedlings inoculated with Erysiphe graminis f. sp. hordei, the causal agent of powdery mildew disease. Iranian Journal of Agricultural Sciences, 31: 415-426.
Quiroga, M., C. Guerrero, M. A. Botella, A. Barceló, I. Amaya, M. a. I. Medina, F. J. Alonso, S. M. de Forchetti, H. Tigier and V. Valpuesta. 2000. A tomato peroxidase involved in the synthesis of lignin and suberin. Plant Physiology, 122: 1119-1128.
Rani, C. I., D. Veeraragavathatham and S. Sanjutha. 2008. Analysis on biochemical basis of root-knot nematode (Meloidogyne incognita) resistance in tomato (Lycopersicon esculentum Mill.) Res. J Agric Biol Sci, 4: 866-870.
Sairam, R. and A. Tyagi. 2004. Physiology and molecular biology of salinity stress tolerance in plants. Current science: 407-421.
Sankar, G. 2017. U.S. Patent No. 9,608,454. Washington, DC: U.S. Patent and Trademark Office.
Singh, S. K. and U. R. Khurma. 2007. Susceptibility of six tomato cultivars to the root-knot nematode, Meloidogyne incognita. The South Pacific Journal of Natural and Applied Sciences, 25: 73.
Suzuki, T., T. Kobayashi, K. Adachi, H. Mochida, H. Iwahori, Y. Tateishi and K. Uesugi. 2012. Effect of Introducing Nematode-Resistant Sweet Potato Cultivars on Crop Productivity and Nematode Density in Sweet Potato-Radish Double-Cropping Systems. Plant Production Science, 15: 48-56.
Taylor, A. and J. Sasser. 1978. Biology, identification and control of root-knot nematodes. North Carolina State University Graphics, 111.
Tutt, A., Robson, M., Garber, J. E., Domchek, S. M., Audeh, M. W., Weitzel, J. N., & Wardley, A. 2010. Oral poly (ADP-ribose) polymerase inhibitor olaparib in patients with BRCA1 or BRCA2 mutations and advanced breast cancer: a proof-of-concept trial. The Lancet, 376(9737), 235-244.
Whitehead, A. G. and J. R. Hemming. 1965. A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Annals of Applied Biology, 55: 25-38.
Wynn, T. A., Chawla, A., & Pollard, J. W. 2013. Macrophage biology in development, homeostasis and disease. Nature, 496(7446), 445.
Yu, Q., & Rengel, Z. 1999. Micronutrient deficiency influences plant growth and activities of superoxide dismutases in narrow-leafed lupins. Annals of Botany, 83(2), 175-182.
DOI: https://doi.org/10.33866/phytopathol.031.01.0476
Refbacks
- There are currently no refbacks.
Copyright (c) 2019 Majid Shahi Bajestani, Esmat Mahdikhani Moghadam, Reza Aghnoum, Hamid Rohani
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
 |  |  | ||
| Pakistan Journal of Phytopathology ISSN: 1019-763X (Print), 2305-0284 (Online). © 2013 Pak. J. Phytopathol. All rights reserved. | ||||