MOLECULAR IDENTIFICATION BASED ON COAT PROTEIN SEQUENCES OF THE BARLEY YЕLLOW DWARF VIRUS FROM UZBEKISTAN

Makhmudov T. Khalimovich, Kadirova Nasirovna, Adilov B. Shukhratovich, Abdikarimov B. Qurombayevich, Abduvaliev B. Abdurashitovich, Ziyaev Z. Mashrabovich, Sherimbetov A. Gulmirzayevich, Kurganov Sardor

Abstract


Wheat has long been considered an important plant in the national economy, and several phytopathogenic viruses have been studied to infect this plant, the most common of which is Barley yellow dwarf virus. BYDV causes great economic damage in agriculture. This article presents the results of research conducted on the BYDV-PAV strain isolated from Uzbekistan. As a result of the conducted research, plant samples (leaf, root, stem) collected from wheat fields in Tashkent region were diagnosed by BYDV-PAV RT-PCR method using special primers based on nucleotide sequence of ORF3 gene of BYDV-PAV coat protein. As a result of research, the presence of the BYDV-PAV strain was found in the collected samples, and the nucleotide sequence of BYDV-PAV was studied. BLASTN and phylogenetic analysis of the virus isolate based on the determined nucleotide sequence showed the highest genetic similarity by 99.81% with the Morocco PAV-type isolate MA9501 (AJ007929.1) while the lowest similarity rate was 87.10% with the Tunisian isolate PAV-TN4 (JX402456.1).


Keywords


Wheat, RT-PCR, Cloning, Molecular characterization, Phylogenetic analyses.

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References


Bencharki, B., J. Mutterer, M. E. Yamani, V. Ziegler-Graff, D. Zaoui and G. Jonard. 1999. Severity of infection of Moroccan barley yellow dwarf virus PAV isolates correlates with variability in their coat protein sequences. Annals of Applied Biology, 134: 89-99.

Choudhury, S., D. Al-Shammari, H. Hu, H. Meinke, G. Westmore, C. Birchall, P. Larkin and M. Zhou. 2018. A screening method to detect BYDV-PAV resistance in cereals under glasshouse conditions. Plant Pathology, 67: 1987-1996.

Close, R. C. and A. I. Tomlinson. 1975. Dispersal of the Grain Aphid Macrosiphum miscanthi from Australia to New Zealand. New Zealand Entomologist, 6: 62-65.

Deligöz, İ., Y. K. Caner and H. Akyol. 2011. Preliminary investigations of Barley yellow dwarf virus-PAV and Barley yellow dwarf virus-MAV in wheat fields of Samsun and Amasya Provinces in Turkey. Plant Protection Bulletin, 51: 187-193.

El-Muadhidi, M. A., S. Murad, M. Jerjess, K. M. Makkouk and S. G. Kumari. 2001. Survey for legume and cereal viruses in Iraq. Survey for legume and cereal viruses in Iraq, 10: 1-10.

Erkan, E. and N. Yılmaz. 2009. Determination of virus diseases on wheat growing areas of Samsun province. Anadolu Tarım Bilimleri Dergisi, 24: 67-75.

FAO 2018. Preliminary data now available for selected countries and products. Erişim Tarihi. Retrieved 2018 March 03 from http://www.fao.org/statistics/en/

Fayziev, V., D. Jovlieva, U. Juraeva, J. Shavkiev and F. Eshbоev. 2020. Effects of PVXN-UZ 915 necrotic isolate of potato virus X on amount of pigments of Datura stramonium leaves. Journal of Critical Reviews, 7: 400-403.

Lorenz, M. W. 2009. Migration and trans-Atlantic flight of locusts. Quaternary International, 196: 4-12.

Makkouk, K. M., S. G. Kumari, Z. Kadirova and A. Zueva. 2001. First Record of Barley yellow striate mosaic virus and cereal yellow dwarf virus-RPV Infecting wheat in Uzbekistan. Plant Disease, 85: 1122-1122.

Margaritopoulos, J. T., L. Kasprowicz, G. L. Malloch and B. Fenton. 2009. Tracking the global dispersal of a cosmopolitan insect pest, the peach potato aphid. BMC Ecology, 9: 13-13.

Nancarrow, N., F. E. Constable, K. J. Finlay, A. J. Freeman, B. C. Rodoni, P. Trebicki, S. Vassiliadis, A. L. Yen and J. E. Luck. 2014. The effect of elevated temperature on barley yellow dwarf virus-PAV in wheat. Virus Research, 186: 97-103.

Parizoto, G., A. Rebonatto, J. Schons and D. Lau. 2013. Barley yellow dwarf virus-PAV in Brazil: seasonal fluctuation and biological characteristics. Tropical Plant Pathology, 38: 11-19.

Ramazonov, B., K. Mutalov, V. Fayziev and S. Koraev. 2020. Morphogenetic characteristics and biological activity of takyr and meadow soils of the republic of karakalpakstan (on the example of soils of chimbay district) morphogenetic characteristics and biological activity of takyr and meadow soils of the republic of karakalpakstan (on the example of soils of chimbay district). Journal Of Critical Reviews, 7: 243-249.

Rybicki, E. P. 2015. A Top Ten list for economically important plant viruses. Archives of Virology, 160: 17-20.

Siddiqui, N. N., M. Ilyas, S. Mansoor, A. Azhar and M. Saeed. 2011. Cloning and phylogenetic analysis of coat protein of barley yellow dwarf virus Isolates from different regions of Pakistan. Journal of Phytopathology, 160: 13-18.

Sobirova, Z., V. Fayziev and K. Abduraimova. 2020. Effect of the virus of the yellow dwarf corn mosaic growth and development of varieties of corn in various phases. Journal of Advanced Research in Dynamical and Control Systems, 12: 602-606.

Sun, J. X., Q. Li, X. l. Tan, J. Fan, Y. Zhang, Y. G. Qin, F. Francis and J. I. Chen. 2022. Population genetic structure of Sitobion miscanthi in China. Journal of Integrative Agriculture, 21: 178-187.

Sunnatovich, K. T., K. Z. Nasirovna, D. K. Sotvoldievich and A. Z. Rakhmatovna. 2020. Analysis of lysimetric parameters of the effect of chemical and biological drugs against Alfalfa Mosaic Virus. The American Journal of Agriculture and Biomedical Engineering, 02: 25-29.

Tamura, K., G. Stecher and S. Kumar. 2021. MEGA11: Molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38: 3022-3027.




DOI: https://doi.org/10.33866/phytopathol.035.01.0875

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Pakistan Journal of Phytopathology
ISSN: 1019-763X (Print), 2305-0284 (Online).
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